I am current using the SRB assay to assess cytotoxicity of 2 drugs and then running median effect analysis to calculate cytotoxic interaction (ie synergy/antagonism). However, I'm now running into the problem that i'm trying to knockdown a gene with siRNA and then seeing if this has an effect on the interaction. The siRNA itself has a slight growth inhibitory effect. Is it possible to treat the transfected cells as a different cell line and just run the combination index analysis with the 2 drugs, or do I have to somehow take into account the siRNA effect in the calculation? I am also planning to use a third drug instead of the siRNA, and that drug has no effect as single agent.
I would really appreciate some advice from anyone familiar with this scenario or even references to any articles that have done what I'm trying to do.
Combination Index help
No replies to this topic