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Standard Curve - Why does the blank have an OD value?


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#1 Agnete

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Posted 10 June 2010 - 12:19 AM

I have a couple of questions regarding ELISA standards. Is the curve supposed to be of sigmoidal shape? and the blank at 0 has an OD value of 0.1 - is that normal?

#2 leelee

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Posted 10 June 2010 - 12:55 AM

For the second part of your question- yes it is normal for the blank to have an absorbance reading. This is your background absorbance (ie absorbance of your blank solution) and should be subtracted from each of your test values.

#3 Prep!

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Posted 10 June 2010 - 01:00 AM

generally ELISA does not essentially follow the beer lamberts law... so they best curve to use to analyze a ELISA is the 4 PL graph!!

Edited by Prep!, 10 June 2010 - 01:02 AM.

Support bacteria - They are the only culture some people have!!!
Cheers!!!




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