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Dilution confusion!!!

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#1 Prep!

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Posted 09 June 2010 - 10:25 PM

Hi folks.. this can be a very irritating question but then it has been eating me up since morning so I have to take it out.

If I add 1 ml solution A to 1ml solution B I diluted it 2 times. So what do I say in ratios? 1:1 or 1:2 dilution????!!!!!!!

Similarly, if I added 1 ml solution A to 3 ml solution B I diluted it 4 times. What do I say 1:3 times or 1:4 dilution???!!!!

One of those days in science that people dread to have!!!!
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#2 moljul

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Posted 09 June 2010 - 10:51 PM

Hi folks.. this can be a very irritating question but then it has been eating me up since morning so I have to take it out.

If I add 1 ml solution A to 1ml solution B I diluted it 2 times. So what do I say in ratios? 1:1 or 1:2 dilution????!!!!!!!

Similarly, if I added 1 ml solution A to 3 ml solution B I diluted it 4 times. What do I say 1:3 times or 1:4 dilution???!!!!

One of those days in science that people dread to have!!!!

you know: 1:10 = 1+9

therefore -> 1:4 = 1part +3parts (e.g. 2,5ml + 7,5ml)

and -> 1:2 = 1+1 (in that case some say 1:1, but it is not the adequate designation!)

Edited by moljul, 09 June 2010 - 10:52 PM.

#3 Prep!

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Posted 09 June 2010 - 10:53 PM

Hi folks.. this can be a very irritating question but then it has been eating me up since morning so I have to take it out.

If I add 1 ml solution A to 1ml solution B I diluted it 2 times. So what do I say in ratios? 1:1 or 1:2 dilution????!!!!!!!

Similarly, if I added 1 ml solution A to 3 ml solution B I diluted it 4 times. What do I say 1:3 times or 1:4 dilution???!!!!

One of those days in science that people dread to have!!!!

you know: 1:10 = 1+9

therefore -> 1:4 = 1part +3parts (e.g. 2,5ml + 7,5ml)

and -> 1:2 = 1+1 (in that case some say 1:1, but it is not the adequate designation!)

tat precisely is my confusion.. how to represent such dilutions... can it be represented in ratios?? or just say 2 times of 4 times diluted!!!??!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#4 moljul

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Posted 09 June 2010 - 11:37 PM

Hi folks.. this can be a very irritating question but then it has been eating me up since morning so I have to take it out.

If I add 1 ml solution A to 1ml solution B I diluted it 2 times. So what do I say in ratios? 1:1 or 1:2 dilution????!!!!!!!

Similarly, if I added 1 ml solution A to 3 ml solution B I diluted it 4 times. What do I say 1:3 times or 1:4 dilution???!!!!

One of those days in science that people dread to have!!!!

you know: 1:10 = 1+9

therefore -> 1:4 = 1part +3parts (e.g. 2,5ml + 7,5ml)

and -> 1:2 = 1+1 (in that case some say 1:1, but it is not the adequate designation!)

tat precisely is my confusion.. how to represent such dilutions... can it be represented in ratios?? or just say 2 times of 4 times diluted!!!??!!

i think i donīt really understand what youre problem is and particularly why it is that important!

#5 Prep!

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Posted 09 June 2010 - 11:57 PM

good one.. i guess that explains my state of mind right now!!!!
was just trying to figure out how can i represent dilutions in ratios

essentially if 1:10 = 1 part + 9 parts, then 10 does not represent B!!!!
10 represents the total so its the ratio of A to total... i was wondering is it tat way or is ratio the ratio of A to B!!!
Hope i m clear now!!
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#6 HomeBrew

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Posted 10 June 2010 - 12:39 AM

I have always thought of it as x parts of solute in x parts of total solution. Thus, 1 ml solution A (the solute) to 1ml solution B (the solvent) is a 1:2 dilution. Similarly, 1 ml solution A to 3 ml solution B is a 1:4 dilution, and results in 4 ml of a solution comprising one part of solution A for every three parts of solution B.

Think of it as "parts per total volume".

#7 leelee

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Posted 10 June 2010 - 12:49 AM

Well great! Now I'm confused!!

I had always thought of it as x amount of A for every x amount of B. As ratio of A to B, rather than amount of A in B.
So 1ml of A added to 1ml of B was a 1:1 ratio.

Or I would say A was diluted by a factor of 2 in B.

#8 Chakchel

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Posted 10 June 2010 - 12:54 AM

Don't mix dilution and ratio...

Ratio means you have x parts of A and y parts of B.

x : y

Dilution refers to the total volume.

x : (x+y)

#9 Prep!

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Posted 10 June 2010 - 12:55 AM

well tat is wat brought me to this confusion at the first place hb.. mathematically a ratio is always between A and B and not between A and the total.. please correct me if i understand this wrong!!!
So this parts of A to the total cannot be termed ratio (the mathematical one). i get ur point thoguht.. i have also been using 1:2 and 1:4 till now but then suddenly today morning i had a greater vision may be!!!
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#10 Prep!

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Posted 10 June 2010 - 12:57 AM

Don't mix dilution and ratio...

Ratio means you have x parts of A and y parts of B.

x : y

Dilution refers to the total volume.

x : (x+y)

well iguess i can buy that cause tat is the theory i have been supporting till date... so a dilution can never be represented in ratios!!! hope moljul is clear about my problem and its importance too
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#11 Chakchel

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Posted 10 June 2010 - 01:02 AM

Don't mix dilution and ratio...

Ratio means you have x parts of A and y parts of B.

x : y

Dilution refers to the total volume.

x : (x+y)

well iguess i can buy that cause tat is the theory i have been supporting till date... so a dilution can never be represented in ratios!!! hope moljul is clear about my problem and its importance too

You only have to mention it properly...

When you dilute something 1:4 it is a ratio of 1:3.

#12 Prep!

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Posted 10 June 2010 - 01:03 AM

so true!!!!
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#13 moljul

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Posted 10 June 2010 - 01:18 AM

oh yessss!

and a 1:2 dilution is a 1:1 ratio!!!

#14 Prep!

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Posted 10 June 2010 - 02:26 AM

oh yessss!

and a 1:2 dilution is a 1:1 ratio!!!

i feel so relieved!!!!
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#15 pito

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Posted 10 June 2010 - 03:13 AM

nice topic to confuse people

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.