I would like to stain murine T and B lymphocytes with CFSE in order to detect them in vivo by immunofluorescence after iv transfer into host mouse. I would like to know if I should use special procedures to prepare my cryosections (is fixation in acetone OK ? should I fix the tissue cryosections or the spleen sample before embedding it and freezing it at -70 ?) .... Thanks a lot for any advice and suggestion.
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