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Can I IP from media?


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#1 than4

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Posted 09 June 2010 - 04:10 PM

I know that my protein of interest is expressed on the cell membrane, and it is predicted to have a cleavage site in the extracellular domain.
I want to see if my protein is being cleaved and floating around in the media.
I have an antibody that will bind to the extracellular section.

Can I use this antibody to IP the protein from the media like I would from cell lysates or will that not work due to the large volume? Or do I need to do a TCA precipitation or something similar to concentrate the media first?

Thanks

#2 cacandice1982

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Posted 24 June 2010 - 11:38 PM

yes, u can, but it is best to concentrate the media using concentrator before IP, the medium may be too diluted.




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