Hi guys,
Does anyone know how to make Tris-acetate running buffer for SDS-PAGE ?
I use this http://products.invi...=Search-Product
But I wanted to make it myself. Anyone do this?
Thanks, J
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Tris acetate sds buffer
Started by Jaypea, Jun 09 2010 01:03 AM
2 replies to this topic
#1
Posted 09 June 2010 - 01:03 AM
#2
Posted 09 June 2010 - 04:42 AM
Here's the recipe (see here):
NuPAGEŽ Tris-Acetate SDS Running Buffer
The NuPAGEŽ Tris-Acetate SDS Running Buffer (20X) is available from Invitrogen
50 mM Tricine
50 mM Tris base
0.1% SDS
pH 8.24
1. To prepare 500 ml of 20 X NuPAGEŽ Tris-Acetate SDS Running Buffer, dissolve the following reagents to 400 ml ultrapure water:
Tricine 89.5 g
Tris Base 60.6 g
SDS 10.0 g
2. Mix well and adjust the volume to 500 ml with ultrapure water.
3. Store at +4° C. The buffer is stable for 6 months when stored at +4° C.
4. For electrophoresis, dilute this buffer to 1X with water. The pH of the 1X solution is 8.24. Do not use acid or base to adjust the pH.
NuPAGEŽ Tris-Acetate SDS Running Buffer
The NuPAGEŽ Tris-Acetate SDS Running Buffer (20X) is available from Invitrogen
50 mM Tricine
50 mM Tris base
0.1% SDS
pH 8.24
1. To prepare 500 ml of 20 X NuPAGEŽ Tris-Acetate SDS Running Buffer, dissolve the following reagents to 400 ml ultrapure water:
Tricine 89.5 g
Tris Base 60.6 g
SDS 10.0 g
2. Mix well and adjust the volume to 500 ml with ultrapure water.
3. Store at +4° C. The buffer is stable for 6 months when stored at +4° C.
4. For electrophoresis, dilute this buffer to 1X with water. The pH of the 1X solution is 8.24. Do not use acid or base to adjust the pH.
#3
Posted 09 June 2010 - 10:13 AM
wow. Thanks thats great, didn't think invitrogen would do this. thanks.
James
James