Posted 08 June 2010 - 04:51 AM
Posted 08 June 2010 - 06:33 AM
Posted 08 June 2010 - 09:23 AM
Just curious: why you want to do up to 50x ? Do you think after dilutions into 1x or 0.5x , the pH and buffer strength will be less likely to be affected (unless yours is neutral water)?
For 1 Liters of 10x TBE, you need: 108g Tris base, 55g Boric acid, 40mls 0.5M EDTA (pH 8.0)
(I read somewhere in this forum says that prepare 1M EDTA is impossible)
Since you want 50X TBE, I am skeptic if you can really dissolve 540g tris base, 275g Boric Acid, 200ml 0.5M EDTA into a 1 liter solution. Adding 815g powder of tris base and boric acid takes almost a full bottle, and adding 200ml EDTA I'm skeptic about how will it dissolve the tris base and boric acid. which the salt amount is more than the solution. Furthermore, I think you hardly top up dH20 into your bottle.
So,in my humble opinion, to prepare 50x TBE is impossible. If you can do it please let me know how.
Anyway, I was thinking if I prepare 1 liter 50x TBE and I got 100 liter to use... not a bad idea..
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
Posted 09 June 2010 - 05:57 AM
I never trust anything that can't be doubted.
Posted 09 June 2010 - 12:53 PM