I'd like to prepare relatively long probes for EMSA (~60-70 bp). I'm gonna use the PIERCE 3'biotynylation kit for labeling.
As the DNA fragments are rather long, I want to amplify them by PCR instead of synthesizing. Pierce suggest labeling a single stranded DNA piece in stead of a double one.
How do I denaturate the PCR- products afterwords? I guess by increasing temperature to 95 degrees, but then what? How do I prevent their re-naturation?
Or do you know/have any other suggestion?
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