About gene orientation
Posted 07 June 2010 - 05:57 PM
Posted 08 June 2010 - 12:20 AM
in case 2 expression goes in one direction and although there is a terminator after GOI1 there will be read-through transcription from promoter1.
Posted 14 June 2010 - 04:18 PM
I am just wondering if there is a terminator in between, then how a read through transcript is possible originating from promoter 1.
Another question is what if i use same promoter for both transcripts in case 1. Will there be any steric hinderance?