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About gene orientation


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#1 inder

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Posted 07 June 2010 - 05:57 PM

Hi, i just wanna know what is the effect of gene orientation in a recombinant construct on protein synthesis. Consider following two cases.

TER1-GOI-PRO1---PRO2-GOI2-TER2

PRO1-GOI1-TER1---PRO2-GOI2-TER2

#2 pDNA

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Posted 08 June 2010 - 12:20 AM

in case 1 expression will proceed in two directions and therefore read-through transcription from one transcript to the other will be prevented.

in case 2 expression goes in one direction and although there is a terminator after GOI1 there will be read-through transcription from promoter1.

Regards,
p

#3 inder

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Posted 14 June 2010 - 04:18 PM

Hi,
I am just wondering if there is a terminator in between, then how a read through transcript is possible originating from promoter 1.
Another question is what if i use same promoter for both transcripts in case 1. Will there be any steric hinderance?




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