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pH hepes


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3 replies to this topic

#1 moljul

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Posted 07 June 2010 - 04:16 AM

we currently donīt have a pH meter:

I bought HEPES solution, 1 M, pH 7.0-7.6!
if i add NaCl and CaCl2 to this Hepes buffer - will the pH change that much?

for my purpose i need to have a final pH around 7,4!!

thanks for recommendation

#2 Inmost sun

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Posted 08 June 2010 - 06:27 AM

we currently donīt have a pH meter:

I bought HEPES solution, 1 M, pH 7.0-7.6!
if i add NaCl and CaCl2 to this Hepes buffer - will the pH change that much?

for my purpose i need to have a final pH around 7,4!!

thanks for recommendation


probably not; try a calculation program for buffers;

check at least with pH strips

#3 Gerard

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Posted 09 June 2010 - 11:45 AM

With a pKa = 7,55 (20 °C) the buffer has a good buffering capacity therefore adding NaCl and CaCl2 will have almost no effect on the pH.

Edited by Gerard, 09 June 2010 - 11:45 AM.

Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#4 perneseblue

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Posted 09 June 2010 - 08:16 PM

at 1M, no.

But at concentration below 1mM then yes. You will have ion concentration effects.
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