we currently donīt have a pH meter:
I bought HEPES solution, 1 M, pH 7.0-7.6!
if i add NaCl and CaCl2 to this Hepes buffer - will the pH change that much?
for my purpose i need to have a final pH around 7,4!!
thanks for recommendation
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pH hepes
Started by moljul, Jun 07 2010 04:16 AM
3 replies to this topic
#1
Posted 07 June 2010 - 04:16 AM
#2
Posted 08 June 2010 - 06:27 AM
we currently donīt have a pH meter:
I bought HEPES solution, 1 M, pH 7.0-7.6!
if i add NaCl and CaCl2 to this Hepes buffer - will the pH change that much?
for my purpose i need to have a final pH around 7,4!!
thanks for recommendation
probably not; try a calculation program for buffers;
check at least with pH strips
#3
Posted 09 June 2010 - 11:45 AM
With a pKa = 7,55 (20 °C) the buffer has a good buffering capacity therefore adding NaCl and CaCl2 will have almost no effect on the pH.
Edited by Gerard, 09 June 2010 - 11:45 AM.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
#4
Posted 09 June 2010 - 08:16 PM
at 1M, no.
But at concentration below 1mM then yes. You will have ion concentration effects.
But at concentration below 1mM then yes. You will have ion concentration effects.
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