i am currently not that familiar with FACS analysis:
is it possible to test the same sample for apoptosis AND proliferation markers?
could it be that there is a double positive population - e.g. apoptotic cells which express proliferation markers or vice versa??
1. Proliferation and apoptosis by CFSE-AnnexinV-7AAD +surface activation markers (correlates with proliferation, such asCD38, CD69, HLADR,etc)
2. Cell cycle (anyDNA dye compartible)+ proliferation (Ki67 IC) + apoptosis (by DNA contetnt- hypodiploid pike)