high background reading in ELISA
Posted 06 June 2010 - 09:01 PM
I always have problems getting high reading for blank sample. My friend did it with the same buffer and protocol (but different sample), but managed to get good result. Im afraid this could probably bcoz of my skills. Any advice would help me a lot
Posted 08 June 2010 - 10:55 AM
All things being equal...Usual problem is insufficient wash or residual wash remaining in wells during the assay (ie decant and blot vigorously using fresh paper towel between blotting).
Watch your friend next time around and run your test in parallel!
Posted 08 June 2010 - 12:36 PM
Also, make sure your settings for wavelength, etc. are correct.
Best of luck!