2 replies to this topic

[anonymous]

Hi, RajeshPlease, take your Balb/c mouse and injected into its abdomen cavity of 0.5ml pristane (Sigma) after this of 1 to 2 week reinjected by similar route 1*10(6) your hybridoma cells and to 14-21 days aspirated the ascitic fluid containing essential mAbs.This is all.Best Research to YouDmitriy

[anonymous]

hai ,can some one give me a protocol for hybridomona production in peritonium in mice.please send me soonthank yourajesh

[anonymous]

Hi Dimitriy,I usally produce hybridoma in vitro. Could you give me more detail the method to produce hibridoma in Bal B/c peritoneal? I want to produce mAb to bovin type II collagen.wish your help.