2 replies to this topic

[vigri]

Hi all,
I am working with cell line and looking at a particular protein which has 25 protein coding isoforms from a wide range of molecular weight.

Can anyone tell me if there is some max possible isoforms co-expressed in one particular cell line or is it really possible to have all of them, 25, in only one cell lyne?

My western blots look like zebra stripes and I am quite confused what to explain to my supervisor.

Any help will be appreciated.

[Prep!]

why not? proteins like EPO express all the 14 isoforms (rather glycoforms) in the cell culture harvests!!! but they are same molecular weight!!!
can u explain more??

[Radish]

yup, that happened to me before lol

I had to tag the different isoforms and work with them one by one to make any sense of it lol.

Pure horror that project.