too many isoforms expressing proteins
Posted 06 June 2010 - 11:12 AM
I am working with cell line and looking at a particular protein which has 25 protein coding isoforms from a wide range of molecular weight.
Can anyone tell me if there is some max possible isoforms co-expressed in one particular cell line or is it really possible to have all of them, 25, in only one cell lyne?
My western blots look like zebra stripes and I am quite confused what to explain to my supervisor.
Any help will be appreciated.
Posted 24 January 2011 - 07:35 PM
can u explain more??
Posted 26 January 2011 - 11:52 AM
I had to tag the different isoforms and work with them one by one to make any sense of it lol.
Pure horror that project.