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too many isoforms expressing proteins


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#1 vigri

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Posted 06 June 2010 - 11:12 AM

Hi all,
I am working with cell line and looking at a particular protein which has 25 protein coding isoforms from a wide range of molecular weight.

Can anyone tell me if there is some max possible isoforms co-expressed in one particular cell line or is it really possible to have all of them, 25, in only one cell lyne?

My western blots look like zebra stripes and I am quite confused what to explain to my supervisor.

Any help will be appreciated.

#2 Prep!

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Posted 24 January 2011 - 07:35 PM

why not? proteins like EPO express all the 14 isoforms (rather glycoforms) in the cell culture harvests!!! but they are same molecular weight!!!
can u explain more??
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#3 Radish

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Posted 26 January 2011 - 11:52 AM

yup, that happened to me before lol

I had to tag the different isoforms and work with them one by one to make any sense of it lol.

Pure horror that project.




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