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The use of hygromycin

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2 replies to this topic

#1 erica



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Posted 06 June 2010 - 05:32 AM

I have problem regarding the hygromycin resistant marker. In my vector, there are 2 resistant markers, one of them is hygromycin. I transformed the vector into E.coli and select the bacteria by using selection media with hygromycin antibiotic. May I know isn't alright use hygromycin to select transformed E.coli cells? Or the hygromycin antibiotic is only use to select the transformed plant tissue cells?

#2 blackmouse



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Posted 09 June 2010 - 12:31 AM

hygromycin is usually for mammalian cells...what's the other selection marker?
You might want to try that one...

#3 perneseblue


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Posted 09 June 2010 - 07:52 PM

However, if you are willing to accept the monetary cost and have the appropriate prokaryotic promoter to drive the hph gene, hygromycin can be used against e coli. Hygromycin is normally used at a concentration of 50 μg/ml in Low Salt LB Agar adjusted to pH 8.

I think you need to look at both markers and their associated promoter within your vector. I have a feeling, that you will find that one marker is for prokaryotic selection and the other for eukaryotic selection.
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