The use of hygromycin
Posted 06 June 2010 - 05:32 AM
Posted 09 June 2010 - 12:31 AM
hygromycin is usually for mammalian cells...what's the other selection marker?
You might want to try that one...
Posted 09 June 2010 - 07:52 PM
I think you need to look at both markers and their associated promoter within your vector. I have a feeling, that you will find that one marker is for prokaryotic selection and the other for eukaryotic selection.