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DNA extraction


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#1 shoninxiode

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Posted 05 June 2010 - 12:40 PM

I extract DNA from human stool samples with QIGEN method, most of time are good, the ratio of A260/280 are about 1.7 - 2.0, but a couple of times I got negative reading in A260 or A280 or both, what's wrong?
thanks a lot

#2 Adrian K

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Posted 07 June 2010 - 09:26 PM

most probably you dont get the DNA, or is too low for the detection range.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434




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