Posted 04 June 2010 - 04:03 PM
Posted 04 June 2010 - 11:03 PM
Posted 05 June 2010 - 03:16 AM
How about you use less amount of renilla vector than firefly in transfection?
are you suspecting that renilla is quenching firefly signal?
Posted 05 June 2010 - 06:38 AM
Posted 05 June 2010 - 08:44 AM
I mean your renilla luc vector is producing strong signal. What renilla vector are you using?
yes, i would like to decrease Renilla signal, but at the same time i would like increased luciferase signal. I think higher renilla signal can quench the signal from firefly luciferase. Is it a possible reason for my low luciferase signal?
I am using pRL vector.
Posted 01 July 2010 - 06:28 AM