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Does medium contain proteins and affect 2D gel result?


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#1 kfszelok

kfszelok

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Posted 03 June 2010 - 11:45 PM

I am going to run 2D gel for secretory protein profile to compare two bacteria that growth in Todd Hewitt broth with yeast extract. ;)

The content of the Todd Hewitt broth:
Infusion from 450g fat-free minced meat 10.0; Tryptone 20.0; Glucose 2.0; Sodium bicarbonate 2.0; Sodium chlorde 2.0; Di-sodium phosphate 0.4.

The content of the yeast extract:
Concentrates of the water-soluble portion of Saccharomyces cerevisiae cells that have been autolyzed. The autolysis is carefully controlled to preserve the naturally occurring B-complex vitamins. Yeast extract is considered a non-animal product and is used extensively for many non-animal formulations for bacterial, fungal, mammalian and insect cell culture. Bacto Yeast Extract is one of the most complete and versatile fermentation bionutrients available. Bacto Yeast Extract, UF is ultra-filtered and specifically designed for
tissue culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it is an ideal substitute for fetal bovine serum. Yeastolates are mixtures of peptides, amino acids, carbohydrates, simple and complex, as well as vitamins. Both Bacto TC Yeastolate and Bacto Yeastolate, Ultra Filtered are used as nutritional supplements for bacterial, insect and mammalian cell cultures.

Do you think this medium would affect the 2D gel imagine if I compare two strains?

Any other minimal medium that is good for running 2D gel?

Many MANT THANKS in advance!!!

Shirley




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