i'm a student and a newbie in the field of cell culture and the problem relates to my thesis project. I would like to test the cell viability of fibrioblasts on a rough surface. Previously light microscopy images has been used and viability has been determined based on the cell's morphology, which for me seems to be a time consuming method and not that reliable! First alternative I thought of was to stain the cell and based on that count the cells, so my first question:
Is there any live and dead stains that can be combined that can be observed in a light microspopy?
This will probably solve the reliable problem, but not the time consuming.. so I thought of an MTT-assay, which will quantify the viable cells. If I am correct? I have two questions here:
Will this method work with fibroblast?
How precise is this method? it is probable that the results of the surfaces tested do not differ that much.
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