Hello Guys, i am sure all have come across pipettes contamination.....What is the best way to destroy DNA incorporated inside the pipettes?
0
5 replies to this topic
#2
-
- Moderator
-
- 1,430 posts
Veteran
Posted 03 June 2010 - 02:07 PM
Use barrier tips, and you won't care about whatever contamination there is, and avoid more in the future.
#3
-
- Moderator
-
- 594 posts
Unlimited ligation works!
Posted 03 June 2010 - 04:22 PM
as phage434 has said use barrier tips.
As for your currently contaminated pipette. I believe you can remove the barrel of the pipettor and soak the barrel in a warm solution of 0.5M HCl. I would leave it to soak overnight. After that soak the barrel in distilled water to remove the acid. Change the water and repeat the soaking several time. Once done, dry the barrel, best to leave it in a 50C incubator/dryer.
Then reassemble barrel to the rest of the pipette.
As for your currently contaminated pipette. I believe you can remove the barrel of the pipettor and soak the barrel in a warm solution of 0.5M HCl. I would leave it to soak overnight. After that soak the barrel in distilled water to remove the acid. Change the water and repeat the soaking several time. Once done, dry the barrel, best to leave it in a 50C incubator/dryer.
Then reassemble barrel to the rest of the pipette.
May your PCR products be long, your protocols short and your boss on holiday
#4
-
- Active Members
-
- 38 posts
Enthusiast
Posted 03 June 2010 - 08:13 PM
You can use the DNAZap spray to degrade residual DNA. Ambion sells it, I think.
#5
-
- Active Members
-
- 48 posts
Enthusiast
Posted 12 June 2010 - 09:10 PM
10% bleach rinse will do the job. I had PCR tested on capillary tubes used for sample transferring.
aquaplasmid
"Finally, total RNA can be extracted from frozen whole blood!"
aquaplasmid
"Finally, total RNA can be extracted from frozen whole blood!"
#6
-
- Active Members
-
- 886 posts
Legendary Graduate Beggar
Posted 14 June 2010 - 04:01 PM
Using Bleach and HCL???
Will it be harmful for the "O-rings" inside the pipette?
Will it be harmful for the "O-rings" inside the pipette?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
