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[grant]

I am using centrifugation to fractionate cytosolic and mitochondrial fractions. I would like to know if there is a cytosolic (and a mitochondrial)-exclusive enzyme that can be assayed in order to make sure that the correct fraction has been obtained

[alchemist]

cytosolic markers Phosphofructokinase, or hexokinase, or even aldolase..for mitochondrial preps...cytochrome C, ornithine transcarbamoylase, pyruvate dehydrogenase