A549 cells not doubling
Posted 02 June 2010 - 07:21 PM
My cells are not doubling! I've tried everything: spiking up to 15% FBS, adding more media than usual, placing a pan of water in the incubator for extra humidity. It seems that every few passages they just give up on dividing and just sat there, not dying but not really living either. I mean what's the point of living when you're not doubling, especially when YOU'RE A CANCEROUS CELL! I'm really frustrated... has anyone been in the same situation, or have any idea what else I should try?
Posted 03 June 2010 - 04:29 PM
Posted 05 June 2010 - 06:30 AM
Edited by Mycoplasma, 05 June 2010 - 06:32 AM.
Posted 06 June 2010 - 11:55 PM
Posted 09 June 2010 - 04:18 AM
Hi! I also think that Mycoplasma could be the reason. There is a very quick and not too expensive kit from Lonza (MycoAlert). You only need a plate reader and around 20 minutes.