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Centrifuging resins

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#1 mcb56



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Posted 02 June 2010 - 09:24 AM

Hello I have a question about centrifuging various types of resins. I am binding protein to an amylose resin and I quickly spin it down prior to protein incubation to wash the resin several times. Is there a limit to how long or fast the resin can be spun down without damaging it? What about when the protein is bound? Can a spin it too fast so that the protein elutes off the resin?

Thank you.

#2 mdfenko


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Posted 02 June 2010 - 12:36 PM

which resin. different resins have different strengths. the danger in spinning resins is that you may crush the bead.

you can spin at low speed (<3000 rpm) to pellet.
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