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Canine Lymphocyte Isolation


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#1 kguanciale

kguanciale

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Posted 02 June 2010 - 07:42 AM

I am trying to isolate canine PBMCs using Histopaque. I've tried two techniques: one using a single layer of Histopaque 1.077, and one with a double layer of 1.077 and 1.119. Both times, I only yielded about 60% lymphocytes in isolation. Any suggestions?




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