I am trying to isolate canine PBMCs using Histopaque. I've tried two techniques: one using a single layer of Histopaque 1.077, and one with a double layer of 1.077 and 1.119. Both times, I only yielded about 60% lymphocytes in isolation. Any suggestions?
Submit your paper to J Biol Methods today!
Canine Lymphocyte Isolation
No replies to this topic