Contamination in SH-SY5Y cultures, mycoplasma?
Posted 02 June 2010 - 01:16 AM
I have a contamination in my cultures which shows as a lot of black dots in the cells cytoplasm. In some dishes, also the media contains moving black dots. It also seems as the cells are growing at a slower rate than usual. Could this be mycoplasma? If so, I would not be able to see the bacteria as black dots in the microscope would I? I have tried to grow the bacteria on blood-agar plates but no colonies showed after 4 days. Gram-staining of pellet from the contaminated media did no show any distinct bacteria. I have also tried to throw away everything (cells, media, serum etc) and start with freshly thawed cells and new solutions but the black dots came back after a few days. What could this possibly be?
Posted 02 June 2010 - 04:22 PM
Posted 06 June 2010 - 10:22 PM