6 replies to this topic

[Moondust]

Hi,

Anyone knows the difference between c.p phenol and phenol? C.p phenol is one of the ingredient to make
carbol-xylene , a clearing agent for stain. I'm trying to make the solution up but not sure what c.p stands for.
Tried googling but to no avail.


Thanks!

[Prep!]

Moondust, on Jun 2 2010, 02:02 PM, said:

Hi,

Anyone knows the difference between c.p phenol and phenol? C.p phenol is one of the ingredient to make
carbol-xylene , a clearing agent for stain. I'm trying to make the solution up but not sure what c.p stands for.
Tried googling but to no avail.


Thanks!

is it chlorinated phenol?? i mean tat is the first thing tat came to my mind!!!  

[Prep!]

or this

http://en.wikipedia.org/wiki/CP_55,940

[Trof]

IMHO Chemically Pure

Like this C.P Acetone

[klinmed]

Moondust, on Jun 2 2010, 10:32 AM, said:

Hi,

Anyone knows the difference between c.p phenol and phenol? C.p phenol is one of the ingredient to make
carbol-xylene , a clearing agent for stain. I'm trying to make the solution up but not sure what c.p stands for.
Tried googling but to no avail.


Thanks!

For the purpose of making a clearing solution there is no difference. Carbol-xylene is usually prepared by dissolving 125g phenol in 375 ml xylene. You can use any standard reagent grade phenol.

As for the "CP", it could mean that the material used in the original formulation was of a purity defined by the Chinese Pharmacopoeia. Maybe your recipe comes from an asian publication?   CP is a standard abbreviation and is used in a similar manner as USP (which is a publication that describes approved chemicals/drugs in the United States).

Hope this helps.

[Moondust]

Thanks KlinMed for ur recipe... and the rest who has responded. From your replies, I would gather that it is Chemically Pure (ref..Trof, 2010   ).
I actually got the method from Sass, 1958 ( fastgreen and safranin stain protocol). Thanks all again!

[Moondust]

Could someone also explain to me what is the function of cp phenol (carbo-xylene)? As in what is the function of a clearing agent?
To get rid of water?

This is my protocol and I was thinking of skipping step 6 but before doing so would like to know how essential it is.

1. Stain in aqueous safranin 1-12hr (time depends on specimen) – Try 5 mins, 30mins
2. Rinse in water till no safranin bleeds from specimen
3. Dehydrate rapidly through 30, 50, 70 to 95% (1 min each)
4. Stain in fast green fro 5-30 Secs
5. Dehydrate rapidly through 2 X 2mins changes of absolute ethanol
6. Transfer to carbol-xylene (1 vol of melted c.p.phenol and 3 volumes of xylene) for 5 min ( 1:1 methyl salicylate: xylene)
7.Clear in 100% xylene
8. Mount in synthetic mountant

Thanks heaps for any help.