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satbility of DMEM media


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#1 sara.r

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    sara.r

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Posted 31 May 2010 - 11:08 PM

Hi
we usually made our DMEM medium from powdered form (gibco) and after filteration store it at +4 for 1-2 months, but I dont know if we store this medium for longer time periods like 3-5 months without loss of nutrients. is there any sugesstions?

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#2 bob1

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Posted 01 June 2010 - 05:21 PM

There are labile components in medium, of which only L-glutamine seems to be commonly replaced. The pre-made media available from Invitrogen has a expiry about a year after purchase, so I don't think there will be too much problem with storing yours for longer, so long as you add L-glut.




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