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Cryopreservation


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#1 Born free

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Posted 31 May 2010 - 10:33 PM

Hi!

Can sme1 let me know an alternate to Mr. Frosty. I want to use some other inhouse material as an alternative. Is there some way? Plz let me know!

#2 leelee

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Posted 01 June 2010 - 12:16 AM

We use those foam racks your 15ml centrifuge tubes come in. Put the cryovials in and then turn another rack upside down on top to sandwich them in. -80 overnight (or over the weekend!) and then into LN2 the next day.
Never had a problem with it.

And a lab mate of mine uses those plastic eppie racks, with no problems either.

Hope that helps.




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