2 replies to this topic

[NemaToStella]

Hi there,
in order to concentrate DNA I need to do an isobutanol extraction followed by a diethyl ether extraction. I'm a little puzzled as to whether I should use BioUltra grade isobutanol that has been tested negative for DNAse/RNase or whether I can use the significantly cheaper isobutanol (what are the odds that DNAse survives isobutanol)?
Does anyone have any ideas on that?
Cheers,

NemaToStella

[bob1]

You should use molecular biology grade reagents to enure that you are not contaminating your samples with DNA that may be in lower grade reagents.

[NemaToStella]

Hm, I figured... thanks Bob!