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Isobutanol extraction of DNA


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#1 NemaToStella

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Posted 31 May 2010 - 09:31 AM

Hi there,
in order to concentrate DNA I need to do an isobutanol extraction followed by a diethyl ether extraction. I'm a little puzzled as to whether I should use BioUltra grade isobutanol that has been tested negative for DNAse/RNase or whether I can use the significantly cheaper isobutanol (what are the odds that DNAse survives isobutanol)?
Does anyone have any ideas on that?
Cheers,

NemaToStella

#2 bob1

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Posted 31 May 2010 - 04:05 PM

You should use molecular biology grade reagents to enure that you are not contaminating your samples with DNA that may be in lower grade reagents.

#3 NemaToStella

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Posted 02 June 2010 - 03:03 PM

Hm, I figured... thanks Bob!




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