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TCR alpha probe/primer


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#1 anonymous

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Posted 01 February 2001 - 10:00 PM

Hi,

I want to look in a population of thymocytes if TCR alpha rearrangement has occured and if al TCR alpha mRNA is produced.

I am looking for one of the following:

- a Southern probe that could detect TCR alpha rearrangement

- a Northern probe that could detect a TCR alpha mRNA (i.e. for the constant region Calpha)

- a set of primers that could detect rearrangement at the genomic level or by RT-PCR.

Any suggestion or reference welcome.

Thanks

Francois





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