I want to look in a population of thymocytes if TCR alpha rearrangement has occured and if al TCR alpha mRNA is produced.
I am looking for one of the following:
- a Southern probe that could detect TCR alpha rearrangement
- a Northern probe that could detect a TCR alpha mRNA (i.e. for the constant region Calpha)
- a set of primers that could detect rearrangement at the genomic level or by RT-PCR.
Any suggestion or reference welcome.