Apoptosis assay using fluorescent microscope rather than FACS
Posted 31 May 2010 - 01:44 AM
Posted 01 June 2010 - 12:08 PM
in deed, the same dys are also to use in fluorescent microscopy; Invitrogen offers various protocols for their dyes; you should do live cell imaging
Posted 07 June 2010 - 01:00 AM
Have you tried the APOPercentage kit from Biocolor. It uses light microscopy and is quantifiable.
Posted 08 June 2010 - 06:13 AM
use the same dye, also the FACS staining protocol you can perform for microscopical use. the stained cells can then be brought onto a slide using so called cytospin.
Posted 09 June 2010 - 01:38 AM
Posted 09 June 2010 - 01:44 AM
Posted 23 June 2010 - 03:17 AM