Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

B lymphocyte isolation fromblood


  • Please log in to reply
2 replies to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 28 February 2001 - 10:00 PM

Can someone help me? I need a simple method for B lymphocytes isolation from periferal blood or blood fraction that contains them in greater quantity. Do they separate togerther with leukocytes?Thank you for Your help.

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 08 March 2001 - 10:00 PM

I will test the "Doyle method" for the specifique selection of B lymphocyte. I need more information on it, so..But i can give you the method by magnetic bead, and the reference of the Doyle technic :In vitro development of human monoclonal antibody secreting plasmacytomas. A.Doyle and al, Human Immunology 13 199-209 (1985)

#3 Jay Mone

Jay Mone

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 01 April 2001 - 10:00 PM

B cells can be easily seaparated by first centrifugin whole blood in histopaque-1077. The resulting mononuclear cell fraction contains the B cells. If you need pure cells, you can try panning on petri dishes coated with anti-human IgM, or use magnetic antibodies.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.