2 replies to this topic

[anonymous]

Can someone help me? I need a  simple  method for B lymphocytes isolation from periferal blood or blood fraction that contains them in greater quantity. Do they separate togerther with leukocytes?Thank you for Your help.

[anonymous]

I will test the "Doyle method" for the specifique selection of B lymphocyte. I need more information on it, so..But i can give you the method by magnetic bead, and the reference of the Doyle technic :In vitro development of human monoclonal antibody secreting plasmacytomas. A.Doyle and al, Human Immunology 13 199-209 (1985)

[Jay Mone]

B cells can be easily seaparated by first centrifugin whole blood in histopaque-1077.  The resulting mononuclear cell fraction contains the B cells.  If you need pure cells, you can try panning on petri dishes coated with anti-human IgM, or use magnetic antibodies.