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Nuclear Fractionation

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#1 saik



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Posted 30 May 2010 - 03:40 AM

Hi Everyone!
I am a first year master student.
I am trying to farctionate nuclear order to look at the level of HIF1. I used the Abcam protocol by skipping the measurement of the protein amount with Bradford assay.
Now,I am trying to increase the amount of nuclear fraction,but I dont know how to do?Does anyone have idea?thank you!

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