Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Working with F plasmids

  • Please log in to reply
No replies to this topic

#1 pDNA



  • Awaiting Authorisation
  • PipPipPipPipPipPipPipPipPipPip
  • 494 posts

Posted 28 May 2010 - 08:10 AM

Dear people,

i'm currently trying to work with a mini-F plasmid (never worked with F plasmids before).
Are there some things one has to consider when working with this single copy plasmids?
For example:
1) Are there special protocols available for the isolation of F plasmids? ...or is it sufficient to use spin columns and use the low copy number protocols?

2) Are there special E. coli strains needed for successful cloning with F plasmids? There are some strains that do already contain a F plasmid (like JM109 for example) ...is it possible that E. coli contains two F plasmids? ...what will happen? (we accidentially used JM109 for cloning of a fragment into this F-mini plasmid and got weired results ...therefore i'm interested if this will be a problem due to recombination or if the strange problems are due to some other issue).

Many thanks in advance!
Your suggestions are highly appreciated!


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.