Posted 27 May 2010 - 04:03 AM
why should this measurement normally should be done?
how can i determine the right wavelength to measure my reference??
i dont want to purchase more than one additional absorption filter (e.g. 600nm) for reference measurement of different applications!!
thx in advance
Posted 27 May 2010 - 09:08 AM
If your samples and materials are well defined and the photometer and cuvettes well maintenanced it would not be necessary.
To find your reference wavelenght you have to get a spectrum and look for a wavelenght where the absorbance is low and stable at low and high values from your sample.
Edited by Gerard, 27 May 2010 - 09:10 AM.
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".