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Normal 96-well compatible for luciferase assay?


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3 replies to this topic

#1 WYF

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Posted 27 May 2010 - 02:28 AM

Hi all,

I am about to try measuring luciferase expression with luminometer. I learned that it's best to use the opaque 96-well to prevent the luminescence from affecting adjacent wells. However, i have only the normal transparent 96-well plate in the lab and would like to know if any of you have experience using the normal 96-well plate to read luminescence. Perhaps i could space out the samples?

Thanks!

#2 blue_lotus

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Posted 04 June 2010 - 04:06 PM

Hi all,

I am about to try measuring luciferase expression with luminometer. I learned that it's best to use the opaque 96-well to prevent the luminescence from affecting adjacent wells. However, i have only the normal transparent 96-well plate in the lab and would like to know if any of you have experience using the normal 96-well plate to read luminescence. Perhaps i could space out the samples?

Thanks!



Normal 96 well plate should be fine.

#3 moljul

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Posted 08 June 2010 - 06:08 AM

i did luciferase assay in white/non-transparent plates!!

but i think also black ones are compatible. but NO transparent plates - otherwise luminescence signal from one well can interfere with signal from adjacent well!!

#4 WYF

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Posted 09 June 2010 - 01:13 AM

i did luciferase assay in white/non-transparent plates!!

but i think also black ones are compatible. but NO transparent plates - otherwise luminescence signal from one well can interfere with signal from adjacent well!!


Thanks a lot for the replies!

I tried black 96-well plate the other day. The machine is reading something in wells without anything. The number is very small though around 50 vs 50,000 in wells with my lysates+reagent. But I can imagine higher interference if i were to use a transparent plate.




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