3 replies to this topic

[WYF]

Hi all,

I am about to try measuring luciferase expression with luminometer. I learned that it's best to use the opaque 96-well to prevent the luminescence from affecting adjacent wells. However, i have only the normal transparent 96-well plate in the lab and would like to know if any of you have experience using the normal 96-well plate to read luminescence. Perhaps i could space out the samples?

Thanks!

[blue_lotus]

WYF, on May 27 2010, 02:28 AM, said:

Hi all,

I am about to try measuring luciferase expression with luminometer. I learned that it's best to use the opaque 96-well to prevent the luminescence from affecting adjacent wells. However, i have only the normal transparent 96-well plate in the lab and would like to know if any of you have experience using the normal 96-well plate to read luminescence. Perhaps i could space out the samples?

Thanks!

  

Normal 96 well plate should be fine.

[moljul]

i did luciferase assay in white/non-transparent plates!!

but i think also black ones are compatible. but NO transparent plates - otherwise luminescence signal from one well can interfere with signal from adjacent well!!

[WYF]

moljul, on Jun 8 2010, 03:08 PM, said:

i did luciferase assay in white/non-transparent plates!!

but i think also black ones are compatible. but NO transparent plates - otherwise luminescence signal from one well can interfere with signal from adjacent well!!

Thanks a lot for the replies!

I tried black 96-well plate the other day. The machine is reading something in wells without anything. The number is very small though around 50 vs 50,000 in wells with my lysates+reagent. But I can imagine higher interference if i were to use a transparent plate.