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primers with restriction site


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#1 SF_HK

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Posted 27 May 2010 - 01:26 AM

HI,

I need to design primers with restriction site to clone the entire reading frame of my gene of interest into a vector. I understand I have to add a kozak sequence before the start codon. I know the function of the kzak sequence, but what specific sequence is added? Is it GTC?

#2 TomH

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Posted 27 May 2010 - 02:28 AM

I use ACCATGG where the ATG is the start codon.




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