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total RNA yield


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3 replies to this topic

#1 Carmen

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Posted 26 May 2010 - 10:42 PM

Hi,

I am confused :) . My total RNA yield is increased in my experimental group. Does this mean that the experimental treatment is possibly increasing RNA and protein synthesis?

#2 Muhammad Umer

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Posted 26 May 2010 - 11:21 PM

Would you like to explain a little about the background of your experiment. wat kind of treatment you are giving and what possible outcome you are expecting

#3 jamessmith01

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Posted 27 May 2010 - 02:38 AM

It might just be an indication of higher cell numbers or a better extraction. What are you extracting RNA from - tissue or cells?

#4 Carmen

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Posted 27 May 2010 - 03:03 AM

Hi,

I am extracting RNA from liver tissue. Insulin resistant rats were treated with a compound that improves insulin sensitivity. Insulin stimulates protein synthesis, thus I thought this could possibly explain the increased RNA yield in my treated samples. I used the same weight of tissue and the same RNA extraction protocol for all samples. I do not think it could be due to RNA extraction procedure. The increased RNA yield in treated samples is statistically significant (p<0.0001, unpaired t-test).

Hope this helps,
Carmen




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