After following the protocol for DNA precipitation according to the following protocol, if I do not see any pellet does that mean that I do not have any DNA?
dd 0.1 volume 3 M sodium acetate
add 2.5 volumes 100 % Ethanol
-20°C overnight (+++)
-80°C 1 h (++)
dry ice 15min (+)
spin 20 minutes at 12000 rpm 4°C
carefully pour out / aspirate supernatant (do not lose DNA-pellet)
I used dry ice.I did the precipitation and out of six tubes, only one had a visible pellet. Does that mean all others have no DNA??
Edited by sanu, 26 May 2010 - 01:25 PM.