Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

anion exchange and buffer

  • Please log in to reply
1 reply to this topic

#1 shimshady



  • Active Members
  • PipPipPipPipPip
  • 37 posts

Posted 26 May 2010 - 09:22 AM

I have a biomolecule that im trying to purify using anion exchange (the biomolecule is negative charge). The problem is is that the biomolecule is in phosphate buffer at ph 7.3. Is it alright to put this on an anion exchange column? My thoughts were that it wouldnt bind since the phosphate is negative charge as well. Is this correct? Thanks

#2 mdfenko


    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,202 posts

Posted 28 May 2010 - 10:14 AM

you should have no problem. the phosphate (or other buffering component) will not interfere with the binding of your protein.
talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.