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how to stain suspension culture?


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#1 vitalgene

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Posted 26 May 2010 - 08:45 AM

Hi everyone,

I was going to use K562, HL60, and MOLT4 cells, and wanna make immunostaining(IF), but donnot know how to stain them with antibodies, as they grow in non-adherent way. Please help me out to solve this problem with some protocol.
thank you

v

#2 bob1

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Posted 26 May 2010 - 04:14 PM

I don't have a protocol, but you could try treating them like you would blood and do a smear on a slide.




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