Dialysis into Lipid
Posted 26 May 2010 - 07:40 AM
I have a membrane protein currently solubilised in detergent and I want to replace the detergent with lipid, and have been told the best way to do this is via a slow dialysis. Unfortunately I have been unable to find any protocols on actually how to do this. Can anyone point me in the right direction?
Posted 27 May 2010 - 02:41 AM
Hopefully, after removal of the detergent your protein will not fall out of solution. If the lipid is soluble and MW is less than that of the protein and can pass thru the tubing pores you could use the lipid buffer as your dialysis buffer.
Posted 27 May 2010 - 04:06 AM
I assume the lipids would be soluble, or at least form soluble entities (liposomes or something?) so they would be able to be dialysed in solution.