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want to purchase a fluorimeter


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#1 moljul

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Posted 26 May 2010 - 04:06 AM

hope that one of you could give some recommendation, which fluorophores/filters (with excitation/emission wavelengths) are commonly used?

im am not that sure which filters i should purchase!

thanks in advance

#2 Prep!

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Posted 26 May 2010 - 04:28 AM

hope that one of you could give some recommendation, which fluorophores/filters (with excitation/emission wavelengths) are commonly used?

im am not that sure which filters i should purchase!

thanks in advance


hi moljul it actually depends on the kind of application u do.. suppose u do a intrinsic and extrinsic emossion scan for proein 3D structure filter based machine will not solve your purpose at all!! u might need a monochromator based!!!
moreover if they are filter based make sure that they have many diff ex and em filter ranges so tat wenever u find a new application u can get the filters!!!
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#3 moljul

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Posted 26 May 2010 - 04:42 AM

hope that one of you could give some recommendation, which fluorophores/filters (with excitation/emission wavelengths) are commonly used?

im am not that sure which filters i should purchase!

thanks in advance


hi moljul it actually depends on the kind of application u do.. suppose u do a intrinsic and extrinsic emossion scan for proein 3D structure filter based machine will not solve your purpose at all!! u might need a monochromator based!!!
moreover if they are filter based make sure that they have many diff ex and em filter ranges so tat wenever u find a new application u can get the filters!!!


Prep!, youre so right - a monochromator would solve all the problems, but my boss meant it will be too expensive!!
and now i think, to have filters for FITC,TRICS, Hoechst/DAPI...will the best deal! additionally we will purchase some filters for absorption measurement to cover all applications...




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