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BL21(DE3)LysS competent cells


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#1 samita

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Posted 26 May 2010 - 02:48 AM

I need to prepare BL21(DE3)LysS competent cells, for DH5 alpha I had never used antibiotics. Do for BL21(DE3)LysS I need to used antibiotic CM?????

#2 phage434

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Posted 26 May 2010 - 03:59 AM

Yes, the pLys plasmid has chloramphenicol resistance, and might be lost without selection.




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