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Useful numbers when plating neurons?


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#1 EGF

EGF

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Posted 26 May 2010 - 02:33 AM

Hi,
I need the seeding density of cells necessary to plate mouse primary neurons and a mixed culture of mouse astrocyte and neurons in 12-wells plates (for immunofluorescence) and probably a 75 cm2 flask or 10 cm plate (for western blot). I don't want the cells to die because of low seeding density.
Which is the recommended seeding density for neuronal cells?




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