Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Useful numbers when plating neurons?

  • Please log in to reply
No replies to this topic

#1 EGF



  • Active Members
  • Pip
  • 17 posts

Posted 26 May 2010 - 02:33 AM

I need the seeding density of cells necessary to plate mouse primary neurons and a mixed culture of mouse astrocyte and neurons in 12-wells plates (for immunofluorescence) and probably a 75 cm2 flask or 10 cm plate (for western blot). I don't want the cells to die because of low seeding density.
Which is the recommended seeding density for neuronal cells?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.