I need the seeding density of cells necessary to plate mouse primary neurons and a mixed culture of mouse astrocyte and neurons in 12-wells plates (for immunofluorescence) and probably a 75 cm2 flask or 10 cm plate (for western blot). I don't want the cells to die because of low seeding density.
Which is the recommended seeding density for neuronal cells?
Submit your paper to J Biol Methods today!
Useful numbers when plating neurons?
No replies to this topic