Posted 25 May 2010 - 05:26 PM
Also, has anyone had any luck immunoprecipitating proteins from mouse serum?
Finally, has anyone immunoprecipitated sclerostin (SOST)?
Posted 25 May 2010 - 07:26 PM
Another option is to use a protein-A-HRP conjugate as your secondary. It will bind to IgG in it's native state - so it will bind to the primary antibody you have put on your membrane, but not recognise the leftover antibody from your IP as it should have been denatured by the sample buffer, boiling and SDS-PAGE.
Sorry, I haven't IP'd from mouse serum or for your protein of interest.
Posted 26 May 2010 - 04:53 AM
Thatīs right, you can use it, but you have to mind the IgG.
"This is SPARTA!"
"Iīm the goddamn batman"