So I'm trying to convert a simple OD 600 reading to DNA concentration. For an unknown reason, it just isn't working for me. Please help!
My OD 600 reading is 2.06. I did a 1:100 dilution to obtain it. And I'm just looking to calculate the concentration.
Help me!
~Labrat612
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3 replies to this topic
#2
pito
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Posted 24 May 2010 - 09:58 AM
check this it might be helpfull.
(however: they use the normal wavelength: OD600 is generally used for bacterial cel celturure measurements, not DNA concentration, so I do not understand why you used OD 600
(however: they use the normal wavelength: OD600 is generally used for bacterial cel celturure measurements, not DNA concentration, so I do not understand why you used OD 600
Edited by pito, 24 May 2010 - 10:01 AM.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.
#3
labrat612
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Posted 24 May 2010 - 12:10 PM
Sorry, I meant to type A260/280
Someone else was talking about their wacky OD reading and I typed it.
This just isn't a terrific day for me
Someone else was talking about their wacky OD reading and I typed it.
This just isn't a terrific day for me
Edited by labrat612, 24 May 2010 - 12:11 PM.
#4
rkay447
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Posted 24 May 2010 - 12:25 PM
We've all had those days... but rather than the 260/280 which tells you purity/protein contamination you just need the A260 for concentration.Sorry, I meant to type A260/280
Someone else was talking about their wacky OD reading and I typed it.
This just isn't a terrific day for me
(OD260)*50*dilution=concentration in ug/ul.
