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converting OD 600 reading to DNA concentration


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#1 labrat612

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Posted 24 May 2010 - 09:24 AM

So I'm trying to convert a simple OD 600 reading to DNA concentration. For an unknown reason, it just isn't working for me. Please help!

My OD 600 reading is 2.06. I did a 1:100 dilution to obtain it. And I'm just looking to calculate the concentration.

Help me!


~Labrat612

#2 pito

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Posted 24 May 2010 - 09:58 AM

check this it might be helpfull.
(however: they use the normal wavelength: OD600 is generally used for bacterial cel celturure measurements, not DNA concentration, so I do not understand why you used OD 600

Edited by pito, 24 May 2010 - 10:01 AM.

If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#3 labrat612

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Posted 24 May 2010 - 12:10 PM

Sorry, I meant to type A260/280

Someone else was talking about their wacky OD reading and I typed it.

This just isn't a terrific day for me ;)

Edited by labrat612, 24 May 2010 - 12:11 PM.


#4 rkay447

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Posted 24 May 2010 - 12:25 PM

Sorry, I meant to type A260/280

Someone else was talking about their wacky OD reading and I typed it.

This just isn't a terrific day for me ;)

We've all had those days... but rather than the 260/280 which tells you purity/protein contamination you just need the A260 for concentration.

(OD260)*50*dilution=concentration in ug/ul.




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