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TAP-tag pull down query


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#1 Jaypea

Jaypea

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Posted 24 May 2010 - 08:25 AM

Hi people.

I have my protein of interest TAP-tagged (Calmodulin binding - TEV cleavage - Protein A) I Just wanted to do a simple pull down and then do a western to confirm a protein I know binds the native protein also binds the TAP-tagged version.

So basically I Just want to do one of the steps in the TAP protocol, pull it down using the Protein A section. So protocols I have read have used IgG - beads, the question I have Is could I use Protein G beads to bind nonspecific IgG antibody and then pull down the protein A in the TAP-tag?

Would this work as a solution or should I just buy IgG beads.

Thanks a lot, JP




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