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Can i use agarose gel to seperate 80bp


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#1 Fhannan

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Posted 24 May 2010 - 06:20 AM

Can agarose gel be used to seperate 107bp, 80bp and 25 bp? What percentage, the thing is that i am not comfortable dealing with PAGE, i hope some one came across seperation of 80bp before and every comments are hightly appreciated....

#2 phage434

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Posted 24 May 2010 - 06:26 AM

Nusieve 3:1 agarose or Metaphor agarose will do this, at 2.5 - 3% gel concentration. PAGE will be better.

#3 fishdoc

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Posted 24 May 2010 - 10:14 AM

I have separated 184 bp from 154 bp using a 2.5% gel. Worked well enough to tell the difference. They were separate PCR products, however, not a single sample with both bands in it.




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