Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

starvation question: With o without aminoacids?


  • Please log in to reply
2 replies to this topic

#1 autophagator

autophagator

    member

  • Active Members
  • Pip
  • 27 posts
0
Neutral

Posted 24 May 2010 - 06:03 AM

Hi all.

Usually I use a DMEM medium, with Fetal Calf Serum, non essencials aminoacids and glutamine. So, to starv my U87MG cells I understand that I should use DMEM free of serum, but ONLY free of serum? must I leave the aminoacids in the mixture?

Thanks in advance.

#2 jaya2020

jaya2020

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 79 posts
0
Neutral

Posted 26 May 2010 - 12:56 PM

Hi all.

Usually I use a DMEM medium, with Fetal Calf Serum, non essencials aminoacids and glutamine. So, to starv my U87MG cells I understand that I should use DMEM free of serum, but ONLY free of serum? must I leave the aminoacids in the mixture?

Thanks in advance.



Hi,

Just starve the cells without serum, that will do.

regards

jaya

#3 autophagator

autophagator

    member

  • Active Members
  • Pip
  • 27 posts
0
Neutral

Posted 29 May 2010 - 07:58 AM

Thanks for you advice Jaya.

Bst regards

A.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.